Tomáš Hajkovský

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Questions related from Tomáš Hajkovský

Do you know soil DNA isolation kit that isolates DNA 50-100 kb long?

Hello, I looked through some soil isolation kits but most of them use also mechanical lysis of cells. Do you know about any soil DNA isolation kit giving HMW DNA 50-100 kb long? For example...

12 December 2015 7,592 4 View

How is it possible that after ligation of vector and insert both cleaved by BamHI and electroporation all clones contained only vector without insert?

I work with metagenomic DNA isolated from soil. I isolated cells from soil, purified their DNA on PFGE, electroelution, dialysis, restriction fragmented it with BamHI (checked with EtBr staining),...

10 October 2015 6,060 14 View