We would like to stabilize T-cells in blood while keeping its viability and thus we are trying to avoid products that can fix T-cells in blood. But any suggestions would definitely help.
Thanks!
I have the same question as you have regarding T cells viability and functionnality preservation in whole blodd samples.
I did test on different preservative buffers, and no one of them were helpful.
We tested Stemalpha S0 medium (whole blodd harvested on heparin sodium was diluted in this medium) and we observed no positive impact of this medium on cell preservation (viability or functions).
We showed that we can conserve cells in whole blood only for less than 12hours (after sampling) at room temperature. Conservation of whole blood at +4°C is completely avoid.