Chapat Ludivine

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Questions related from Chapat Ludivine

Which fixative buffer for flow cytometry assays can be used for very fragile cells as primary cells from avian blood ?

I'm actullay working on avian PBMCs, and I've to use a fixative buffer because I can't do the acquisition of my flow cytometry assays at the end of the staining, but when I used PBS + 1%...

10 February 2016 2,327 3 View

Which buffer or reagent should I use for harvesting avain macrophages after 2 or 3 days of culture (so very adherent cells) ?

After 2 or 3 days of culture, I didn't succeed in harvesting live monocytes/macrophages without any compound adding ? I thought that Trypsin will be too agressuive for my cells, Is there other...

10 February 2016 6,164 11 View