You could try the LIVE/DEAD-staining kit from Life Technologies. It uses CalceinA as marker for living cells and Ethidium Homodimer as marker for dead cells. The first is not fluorescent, but gets cleaved into a fluorescent species by an esterase that is only expressed in living cells. The latter becomes fluorescent when it intercalates into DNA, but in order to be able to penetrate into the nucleus, there must be holes in the nuclear membrane, indicating apoptosis.
You can also check the FLICA™ Caspase assays if you are lookink for apoptosis marker. The fixation after the staining is not mandatory. You will have many FLICA kit, depends on which caspase, so apoptotic pathway, you want to study.
For cytofluorimetry, you can use 7AAD which is autofluorescent and intercaletes into DNA, permitting to discriminate died from alive cells. It doesn't require fixation: you have to stain your samples 15 min. before the acquisition. You can read its signal as PerCP fluorochrome.
You can also try the YoPro-1/propidium iodide staining (Vybrant Apoptosis Assay), which differentiates apoptotic and necrotic cells; healthy cells do not stain.
Note that LIVE/DEAD-kit is ok if you want to know at a given moment how cells are doing, but in my experience after a few hours of this cocktail in the medium, most cells are dead.
I am using "ReadyProbes® Cell Viability Imaging Kit, Blue/Green" for adherent cell culture. Additional ReadyProbes Ready-to-Use Imaging Reagents: http://www.thermofisher.com/br/en/home/brands/molecular-probes/key-molecular-probes-products/readyprobes-ready-to-use-imaging-reagents.html
I haven't usd this but there is a caspase luminescence kit that promega sell - https://www.promega.co.uk/resources/protocols/technical-bulletins/101/caspase-glo-37-assay-protocol/
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