Do you know any factor in inactivated serum mediated or related to antigen-antibody reaction in ELISA?

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A bug in SPSS and jamovi?

I was preparing a seminar and accidentally stumbled upon an odd situation. When there is a negative relationship between the predictor (Extraversion) and the dependent variable (Depression...

12 June 2023 7,476 13 View

Cd8a disappearance in FACS, what's going on?

Hi, I'm in a lab for 2 and a half years where we work on mouse tumors. We have a protocol running for 7 years of tumor dissociation, staining, and facsing. We are using the cytoflex lx 5 lasers....

06 June 2023 910 4 View

Can I reinstated zoological nomen nudum (invalid name) to be valid?

Recently, I published two new spider species in Forests and registered two new species in Zoobank after the paper was published. However, the World Spider Catalog currently contains the...

22 March 2023 3,201 2 View

How can I increase the efficiency of Retroviral transduction?

Hi, everyone. I'm master's degree candidate. I research about T cell engineering. I use retrovirus vector, and engineer the T cell through the transduction using retroviral vector. I did...

04 January 2023 6,339 0 View

How can I oxidative degrade the natural rubber?

I try to degradation of natural rubber latex using 20% low ammonia NR latex. "Degradation of NR was carried out by preparing latex with 20% dry rubber content (DRC). The pH of the latex was...

19 June 2022 367 5 View

Error message in Gaussian16 for calculating hyperpolarizability??

Hello! I have a problem in calculating hyperpolarizability using Gaussian16. I put the gif file and input the command -> g16 filename.gif filename.out & Then, an error message pops up and...

24 November 2021 5,403 2 View

How can i use high-k material such as TiO2 in TCAD sentaurus?

Hello I'm new user of TCAD sentaurus. I tried to simulate MIS HEMT using TiO2, but TiO2 was not on the material list. But someone said sentaurus can use TiO2 as high-k materal. How can I use it?...

14 September 2021 4,723 2 View

Is it possible to fabricate a BaSi2 film on the SLG glass substrate using chemical deposition process?

The possibility of semiconducting BaSi2 films for potential application in thin-film solar cells has already been explored by several research groups. Literature review reveals that to grow...

18 May 2021 5,775 5 View

How do you adjust the base layer thickness of agarose gel when electrophoresis in a comet assay?

How do you adjust the base layer thickness of agarose gel when electrophoresis in a comet assay? When I was doing peparation of Slides, dip conventional slides and wipe underside of slide and...

28 December 2020 6,758 2 View

What is the communication between promoter and IRES in viral vector?

hi, I have an simple question related to IRES. Regard with my cloning experiment, I noticed one thing. promoter-gene1-IRES-reporter gene Here is a sequence which I used. In that case, I...

20 December 2020 5,498 2 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How does the Retention time effect the Biogas Production?

List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?

27 July 2024 4,800 4 View

What does negative readings in spectrophotometer denote. Was testing TSS content of bacterial cultures?

I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...

22 July 2024 9,576 4 View

What are the strategies to Enhance IgG-Producing Plasma Cells in mice for Monoclonal Antibody Development?

I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...

22 July 2024 9,160 2 View

How do I prepare a working stock solution of 14C sodium bicarbonate to get a final activity of 3uCi?

I need to make a stock solution for radioisotopic incubations of freshwater sediments to study dark primary productivity but I have some confusion as to how I calculate the concentration of 14C...

15 July 2024 5,898 0 View

Isolation of Hydrogen Producing Bacteria?

any good research which can show selective microbial strains for producing hydrogen? Yes the normal procedure is known but the isolation is the key for such selective microbial strains which can...

13 July 2024 2,255 3 View

Hi. Can I get the list of publishers for microbiology-related short communications without publication fee (APC)?

I need to know which journals are exempt from publication fees (APC) in the area of microbiology

11 July 2024 6,346 2 View

What are the regulatory guidelines to submit a microorganism as Generally Regarded as Safe ?

Give in detail with available literature and website links for submissions

27 June 2024 9,753 1 View