What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

How to calculate ECC using HFSS software?

Good morning. Now i am designing MIMO antenna for Ultraband Applications. Can anyone suggest how to plot ECC (Envelope correlation Coefficient) Using HFSS 15 software. Thanks in advance

13 February 2020 5,261 6 View

What is the zeta potential value of deionised or distilled water?

I am getting zeta potential value of about -30 mV for distilled water obtained from a commercial supplier. What it means? What should be the zeta potential value of distilled water? How can I...

10 October 2017 2,791 19 View

Zeta potential for amine functionalized Nanoparticles?

What will be the sign of zeta potential for amine functionalized nanoparticles? The amines are neutral (not protonated). Due to the electron rich character of amine functional group shall I expect...

30 July 2016 9,866 4 View

How to compare more than 50 groups?

i want compare more than 50 groups? SPSS could not performing ANOVA. which test shal i use to compare these groups ? kindly give your suggestions

05 July 2016 3,389 6 View

What is the statistical methods or test to identify first five best groups from several groups?

I am doing some diversity analysis for mineral content. If i want to compare and identify five best groups/samples from 70 samples (for 10 different parameters-independent variables), which...

30 May 2016 956 3 View

Is the zeta potential value valid for amino acid functionalized nanoparticles?

I have modified my carbon nanoparticle surface with amino acids. Now I would have both amine and acid functionalities on the surface of my nanoparticle. The measured zeta potential value is about...

31 December 2015 6,592 10 View

Can anyone suggest literature on Regional Innovation Systems in solar energy in the Indian context?

26 March 2015 5,749 2 View

What can be used as a standard for alpha amylase and glucosidase activity?

Why we should use acarbose as reference for alpha glucosidase activity? Is there any other standard that can be used for this enzyme assay? Can maltose be used as reference for alpha amylase activity?

07 October 2014 1,297 1 View

DTT or BME - which one is better for RNA isolation from 293T cells?

In a Qiagen RNA isolation kit either DTT or BME has to be added to the RLT buffer.

26 March 2014 4,998 4 View

For a new biofertilizer what efficient methods should I follow to test it's quality(non-presence of pathogens, types &number of symbiotic microbes)?

Also it's effects on plants.

22 December 2020 2,318 3 View

Will the of use of vermicompost as nutrient source in agronomic crops possible ?

We know that vermicompost is far better than compost manure and chemical is too exe and deficit too so for marginalized farmer it will be better to promote vermicompost but its production in...

09 December 2020 1,873 22 View

How to prepare lignin basic media ?

I want to prepare bacterial media containing Kraft lignin ( dissolved in 0.1 M NaOH). after I autoclave,the lignin gets precipitated. Please suggest how to retain the solubility of lignin in the...

07 December 2020 800 3 View

Any simplified protocol or methods of estimation of cellulose, hemicellulose and lignin content in a biomass?

Dear all, Kindly suggest me a simplified/easy method of calculation of cellulose, hemicellulose and lignin content of agricultural biomass along with the relevant reference. We have used the NREL...

02 December 2020 4,362 3 View

How to amplify lignin degrading gene?

Hello colleagues, I would like to amply the lignin-degrading gene (i.e., LigX, LigY, LigZ, and LigW) from different types of soil. I have tried to find literature, but unfortunately, there are...

20 October 2020 9,654 3 View

What is the best procedure to extract bast fibre from plant bark having higher lignin content?

I have tried to extract fiber from the plant bark having higher lignin content with all traditional fiber extraction methods as well as treated up to 20% NaOH Solution at 100 Degree C. However...

04 October 2020 8,149 1 View

Please identify the fungus ?

It was found in plant growing media (soilless + neem caka + vermicompost).

09 September 2020 138 21 View

How to prevent leaks in Swagelok fittings?

I am carrying out a liquefaction reaction inside a 2.5mL volume reactor made of Swagelok fittings (one SS 3/4" STRAIGHT UNION with two SS 3/4" PLUGS FOR FITTING). After adding the substrate...

11 August 2020 4,211 2 View

How to prevent lignin condensation during biomass fractionation?

10 August 2020 4,936 1 View

Can anybody help me to identify this animal?

These (please see 7 photographs in the attachment) worm like animals are found in the vermicomposting ( Eudrilus eugeniae) bed in Bangladesh. I am confused about their identity. I shall be happy...

21 June 2020 2,498 13 View