Do you know a protocol for chemical transformation for Debaryomyces hansenii?

02 February 2014 1 3K Report

I am trying to delete a gene of interest in D.hansenii. There is a protocol for transformation of this yeast by electroporation, but for me it is more convenient to use chemical transformation. Can anyone suggest a relatively efficient protocol for chemical transformation of yeast D. hansenii?

Celia Payen

I don't but ask Serge Casaregola from the Genolevures consortium: http://genolevures.org/about.html#

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