Do you know a good protocol for Thin-Layer Chromatography Bioautography Assay for Plant Extracts to Identify Antimicrobial Compounds
Hi, I think you can search in the Camag TLC Data Base, I found 99 hits with key word "bioautography", I hope you can find suitable methods, Best regards
http://www.camag.com/en/tlc_hptlc/customer_magazine_cbs/ccbs_database.cfm?method=query
Other than making sure the solvents are evaporated from the TLC plates, I would lay the plates on the agar with the culture for a period of time (an hour or 2, if I remember correctly), then remove the plates and incubate overnight. I was using bioautography to determine where/if my compound would elute.
I would run silica and alumina in different solvent systems to determine which might best resolve the anti-microbial compounds from the other material.
Here is a nice overview: http://www.sciencedirect.com/science/article/pii/S2095177914000598
Here is other potentially useful links: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3005382/
http://www.rsi2004.lubelskie.pl/doc/sty5/art/Grzelak_E_art.pdf
The Original protocol is described here: "Nostro, A., Germano, M.P., Angelo, V.D., Marino, A. and Cannatelli, M.A. (2000) Extraction methods and bioautography for evaluation of medicinal plant antimicrobial activity. Lett Appl Microbiol 30, 379–384." with pretty much details!
Please ask me/ message if you have very specific questions during it's implementation. Should be easy implementation.
I have also successfully implemented in my work (attached manuscript!).
Thanks,
Biswa
Plz check the publication. Hope it will be helpful
Article Antibacterial Activities of Dodonaea viscosa using Contact B...
Thank you very much Muhad. Will check the journal article.
Agar diffusion or contact bioautography method seems pretty good because of its sensitivity for plant extracts to detect antimicrobial compounds.
- Badges
- Science topic
- Similar topics
- Biological Science
- Botany
- Pharmaceutical Botany
- Plant Extracts