How do we interpret sf12 PCS and MCS scores?

I always read "the higher the score the better the health", however, it seems to me that it is not true by the way the two summary measures are constructed. Or maybe - highly plausible - I am...

11 January 2021 4,620 1 View

Additional bands on Western blot after protein overexpression? What are the additional products?

Dear Researchers, I cloned the IRF9 coding sequence into plasmid LV073. I used the Gibson assebly (NEBuilder) protocol. I cut the vector in MCS with the enzyme ScaI (blunt ends), amplified my...

26 November 2020 5,080 4 View

How do I create a matrix file from my variant frequency to create a sequence logo?

I would like to create a sequence logo from my variant frequency (short 15bp DNA sequence). So it's not the normal alignment of several sequences but only ONE single sequence where I would like to...

31 March 2020 1,405 8 View

Does somebody know about protocol of retroviruses and adeno-assosiated viruses?

Hello! I have a lot of practice with lentiviruses (shRNA, knock-in, CRISPR, etc.). And now I need to transduce human and mouse cells by retroviruses (knock-in). I recieved plasmid pQCXIP and in...

11 January 2020 1,245 3 View

How to determine aerosol optical depth by using MCS data?

13 November 2019 8,227 1 View

Issue with pgr106 vector?

Dear all, I have been trying to use a pgr106 vector for cloning work. However I am facing many issues when I am trying to digest it in the MCS region. The sites I have selected for cloning is cla1...

29 October 2019 8,418 4 View

What kind of situations that make gene insert not stick to MCS?

I was using pgem t easy to ligate my gene (500bp respectively), blunt end and gel extraction product. The case is : because of lower concentration of DNA template for pcr, so I re-pcr my desired...

18 January 2019 2,623 2 View

What kind of situations that make gene insert not stick to MCS?

I was using pgem t easy to ligate my gene (500bp respectively), blunt end and gel extraction product. The case is : because of lower concentration of DNA template for pcr, so I re-pcr my desired...

31 December 2018 5,759 2 View

Need to understand the data rate for different MCS for 802.11?

I am running 802.11 simulations in NetSim and want to understand how the data rate is determined for different MCS in 802.11. Thank you.

31 December 2018 1,870 4 View

Need to understand the data rate for different MCS for 802.11?

I am running 802.11 simulations in NetSim and want to understand how the data rate is determined for different MCS in 802.11. Thank you.

31 December 2018 4,720 3 View