Hi, for native protein electrophoresis u can prepare tris glycine buffer (note: no SDS) and a dye without SDS (no denaturant) and beta-mercaptoethanol or DTT (no reducing agents). u can cast the gel as same as u cast for SDS-PAGE but w/o SDS. to the sample add the dye, no boiling and load on the gel. for control purpose load BSA appr 5mircogram, u should see 3 bands in BSA.

we r using 6% for 450kDa protein for western blotting. never did native for this protein.

if ur protein is monomer then u can try the same, if dimer or higher oligomer then no idea!!!

good luck