Do you have any suggestions/protocol to perform Native Protein Electrophoresis and Western Blot on a very big protein (400kDa)?

I'm thinking about using 7%Tris Acetate gel and TrisGlycine sample and running buffers with no SDS and no heating for electrophoresis. Plus,TrisGlycineMethanolSDS for transfert on Nitrocellulose...

04 April 2013 1,412 1 View