Thank you for your answer! I do want to use for immuno procedure to check the regenerated axons. Have you ever done with anyone above? Which one is better for my purpose?
I think it depends on the timing of your experiment. If you are looking "early" as in less than 6 weeks GAP-43 should be good. Otherwise a method of anterograde or retrograde tracing may be better.
We used GAP 43 immunostaining, 12 weeks after spinal cord lesion. In our study GAP43 was quite unspecific.We also tried SCG10 which was better than GAP43, but also not a best marker in our case. I do agree with James comments on Gap43 staining and very much preffer anterograde or retrograde tracing.
I agree with the previous suggestions. You may also try something more risky if you feel "entreprenurial": PrPc (cellualr prion protein, the "normal one) SAF-32: ( SPI-CEA) 1/125.
PrPc has been involved in axonal growth and regeneration. Please let me know if you try
Check: J Neurochem., 92:1044-53, 2005 and Neuroscience Vol. 111, No. 3, pp. 533-551, 2002