We want to deposit antibody on specific parts on silicon chip, using dip pen nanolithography(DPN) with AFM. Is antibody be denatured on SiO2 substrate?
I've been using Aspen Plus software to simulate sludge gasification and far I've relied on the information and explanations provided in literature to specify the equipment. However, I'm currently...
05 September 2023 1,206 3 View
Dear all, I am simulationg a CNT in water using Dissipative Particle Dynamics with energy conservation. I can find most of the parameters for Carbon (like repusion parameter...)from the related...
12 May 2023 5,113 3 View
In the article titled "FDOPA-(18F): a PET radiopharmaceutical recently registered for diagnostic use in countries of the European Union", two half-lives are reported for FDOPA....
19 November 2022 7,799 9 View
I have been working on a pain intensity study and I wanted my patients to report their pain intensity using numerical rating scale. many patients have reported thier pain in spectrum like 2 to 3...
13 February 2021 6,809 3 View
In my current research the sample size was calculated to be 500 people. I have gathered the data now I want to cosider my exclusion criteria and exclude 80 of them. therefore only 420 samples...
07 January 2021 3,200 1 View
Dear colleague I am working on U87-MG line. I have seen some new shapes of cells in my two recent subculture that were strange for me. it is passages number 7, i used DMEM high glucose containing...
25 October 2019 3,409 1 View
Are you aware of any reminder apps that can be connected to Qualtrics? I'd like to send daily signals to participants' phones and I want the participants to be able to open the qualtrics survey...
05 May 2019 930 1 View
HI, I am working on vesicular stomatits virus ( VSV) and i should harvest high titer of VSV for about 500 ml. up to now i did TCID50 assay to measure the vsv titer i harvested and i got 10-5...
31 December 2018 3,776 2 View
We are looking for the mechanical characteristics of normal and osteoporotic bone. Anybody can suggest us some references including Young modulus of elasticity, tensile, shear and compressive...
02 December 2018 5,526 3 View
should we wait for a while or we could receive a temporary prosthesis immediately following amputation? What items influence on this timing?
06 October 2017 5,103 5 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
We intend to study the interaction between peptides and polymer (like PP, PE and PS) through MD simulations using Martini force fields ( Martini 2 for PP and Martini 3 for PE, PS). We have...
08 August 2024 4,842 0 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...
07 August 2024 9,563 0 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...
04 August 2024 4,255 1 View
average particle size calculation from TEM
04 August 2024 2,921 1 View
I am working on a SEM model where i have 7 latent variables (6 exogenous and 1 endogenous). In AMOS when I co-vary the exogenous constructs, only 2 paths are coming significant out of 6. But when...
03 August 2024 6,028 4 View
Suppose I am studying the construct of "Academic Motivation" (latent variable) in students, which is measured through three observed variables: "Intrinsic Motivation," "Extrinsic Motivation," and...
31 July 2024 3,913 6 View