Can someone provide me with the assay to determineLC3-Associated Phagocytosis (LAP)? How can I also activate or inhibit LAP?

I am conducting a study where I need to be able to identify LC3-associated Phagocytosis (LAP). I also want to know how to inhibit or activate LAP

10 September 2018 6,371 1 View

What is (are) the best theoretical framework to assess the impact of climate change on food security, livelihood and adaptation strategies?

I want a theoretical framework that can be used to assess the impact of climate change on food security, livelihood and adaptation strategies.

21 March 2018 8,239 11 View

Do E. coli sometimes exceed faecal coliforms in surface water when using MLGA?

10 May 2017 4,145 3 View

Starting of MD simulation of a protein in water on GROMACS

Hello, I am trying to run molecular dynamics simulation of a protein in water in GROMACS. The protein contains 21 subunits(chains). There are three types of subunits. I have attached a GFP to each...

28 November 2016 6,605 4 View

What is the best solvent to dissolve powdered, purified water soluble extracts from plants . Please exclude water itself, DMSO and acids in general?

I am trying to do a TLC on water soluble extracts from some plants

09 October 2016 7,188 7 View

What is the best solvent to dissolve powdered, purified water soluble extracts from plants . Please exclude water itself, DMSO and acids in general?

I am trying to do a TLC on water soluble extracts from some plants

06 October 2016 6,248 6 View

Is it possible to find an explicit example of a midconvex function?

Midconvex functions that are not convex are not Lebesgue measurable

22 January 2016 2,805 3 View

I am trying to identify the genomic structure of some bacteriophages. Can anyone send me a protocol?

Protocol of how to identify bacteriophage genome structure using restriction enzymes. I have Bal31 and SexA1 R.E.

23 April 2014 10,001 2 View

How can the use of Jolly Phonics improves the quality of reading, speaking and writing in rural disadvantaged communities in Papua New Guinea?

Jolly Phonics reading program is an effective way to teach early reading to young learners. How can lectures at the university prepare early childhood student teachers to teach Jolly Phonics to...

03 March 2021 3,731 3 View

Covid 19 Pandemic ' impact on digital transformation?

Covid 19 Pandemic and digital transformation

22 February 2021 3,351 5 View

Which are the most recent implications of ESL Academic Discourse in language teaching?

Language teachers at UTP (Language Department) are increasingly interested in the role of academic papers in the development of language (English) teaching practices. Research papers in Academic...

22 February 2021 1,388 4 View

In CDA analysis we have three stages, viz., description , interpretation, and reproduction. Why have all CDA studies neglected the third stage?

In CDA analysis we have three stages, viz., description , interpretation, and reproduction. Why have all CDA studies neglected the third stage?

18 February 2021 6,483 3 View

Can I follow Researchers from Teaching and testing areas?

I want Researchers from Educational Measurement and Evaluation, relating to teaching, learning , academic performance and test validation

18 February 2021 4,757 13 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

Is the logarithmic transformation a necessary step in time series analysis ?

Hello, I'm checking if the log-tranformation of time series data is a necessary step; We all know the aim of such transformation, but in every study (in time series analysis) we found the authors...

15 February 2021 5,655 14 View

Are you looking for a quality online conference on teaching and learning technology?

This large international conference will be held online this year due to COVID. I am submitting a paper to the Technology Enhanced Language Learning track (8). It is about a topic I have never...

15 February 2021 1,975 15 View

How much amount of DNA (ligation mix) should I use for transformation?

Hi Fellow Scientists! I am trying to clone my 300 bp insert into a 6 kb vector. I run a few different ligation reactions using (1:3 vector: insert molar ratios) 10, 20,30, and 40 ng/ul vector...

15 February 2021 9,563 6 View

How to produce huge amount of protoplasts from Fusarium graminearum?

Hi, all I am using some protocol for protoplast production from Fusarium graminearum (Fg), but I need them in a huge amount. Sometimes the protocol worked well but most of the time failed....

14 February 2021 2,286 4 View