Is there an appropriate CD8 antibody for IHC-P for staining human breast cancer tissues?

Hello, We are planning on staining Formalin-fixed paraffin breast cancer tissue sections (FFPE) with CD8 Ab via immunofluorescence (Tissue IF). Based on our existing protocol and imaging...

24 February 2021 9,578 4 View

How can I get the coding gene sequences of Hela and U2OS cell lines? Is mRNA sequence of Hsp90 alpha tissue specific??

I am looking for hsp90 mRNA sequence in Hela and U2OS cell lines, I have tried through ncbi but always the output is hsp90 in homo sapiens not in the specific cell line. Kindly, could any one...

22 February 2021 1,853 3 View

What is the difference between actin and loading control in normalization of western blot???

Regarding normalization of western blot analysis, I have found different strategies people follow but I couldn't understand the principle and which one is the accurate, 1- some refer to loading...

04 December 2020 5,721 8 View

Detecting bands by ImageJ on mac not fixed properly in each lane???

I am using ImageJ on mac and when I select bands, the first one detected correctly, when I click cmd 2 (= ctr 2 on windows system) to detect the second band, the rectangle gets fixed on the...

01 December 2020 9,538 4 View

My question is about how to determined MIC concentration in the use of microdilution method?

I would like to ask you about MIC first, after I searching in the internet about my query I could not found any answer. My question is about how can I calculate the concentration of stock...

23 November 2020 6,737 3 View

Can you please help me with organic acid HPLC analysis?

Hello everyone, I am experiencing difficulties in the HPLC analysis of adipic acid. I have tried Atlantis C18, mobile phase phosphoric acid solution PH 2.65, flow rate 0.7 ml/min, column temp 35...

20 November 2020 8,280 4 View

Is THC causing underlying paranoia in individuals?

In other words, is there a chemical impact caused by THC which could be activating neural correlates in the insular cortex (ic) of the brain? The increase in the sensory activating...

04 August 2020 967 4 View

Is vitamin C beneficial against Corona virus?

Vitamin C (ascorbic acid) is a water-soluble vitamin that is thought to have beneficial effects in patients with severe and critical illnesses. It is an antioxidant and free radical scavenger that...

04 August 2020 3,856 98 View

How long does the coronavirus stay on your clothes?

03 August 2020 1,674 96 View

Are health workers at risk from a novel coronavirus?

Are health workers at risk from a novel coronavirus? Yes, they can be, as health care workers come into contact with patients more often than the general public WHO recommends that health care...

27 July 2020 1,027 97 View

What are the contents of the supplication of the oppressed that Imam Al-Hadi (peace be upon him) teaches us?

What are the contents of the supplication of the oppressed that Imam Al-Hadi (peace be upon him) teaches us?

08 February 2021 6,791 5 View

Does anybody know about literature on protest and post-conflict processes/societies? What would you recommend?

I am trying to start a project on the subject and your help would be appreciated

18 January 2021 2,190 6 View

What could be the role of researchers to reduce violence in school?

I want to promote peace and non-violence in school and collaborate for such initiative in future.

30 December 2020 973 3 View

What the essence of yoga and meditation ,how you take it as?

we all have our own thought and experience regarding yoga and meditation, we all know it's a miracle unexplainable to those who do he/she feel it.What's your view regarding it.

24 December 2020 3,615 10 View

What is the difference between Micro-Electro-Mechanical Systems, or MEMS and Nano-Electro-Mechanical system or NEMS?

As the technology is growing at very fast pace with many latest advancements. For which the micro level objects were transferred to nanoscale that provides enormous applications. Inspite of all...

26 August 2020 7,172 16 View

Which book do you recommended to read that can bring peace and tranquility to the soul ?

COVID-19 brings a terrible atmosphere everywhere. Books are trusted friend in good and bad times. Some are fond of novels and some are of holy scriptures. In this era of grilling which book make...

25 August 2020 3,203 32 View

Is there any website for blasting primer sequence for Brassica napus other than www.brassicadb.org?

Hello Altruist, I am a newbie in Brassica research. I am working on RFo (Restoration of Fertility of Ogura type MS) marker development for rapeseed. We have some very good SNPs identified from...

26 July 2020 445 2 View

Should there be a Nobel prize in mathematics ?

Six Nobel Prizes are awarded each year, one in each of the following categories: literature, physics, chemistry, peace, economics, and physiology & medicine. However Mathematics a subject mankind...

09 June 2020 8,869 1 View

How to Find True Happiness and Peace in your heart and mind?

Different people have different ways to find happiness and peace in their heart and mind. What is your's way?

08 September 2019 9,979 9 View

How can one be a smart person?

Each one of us work ourselves for our lively hood. Some works hard in his daily routine and some apply the brain to get the work done having got the same income be relaxed and in a peaceful way....

06 July 2019 5,318 39 View