Do we have examples of oxidases that do not follow the ping pong mechanism when 2 substrates are present?

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Does anyone have experience of using thrombin to cleave off the His tag and some adjoining amino acids from a protein?

How does one obtain the required protein by separating out thrombin from it?

10 November 2014 8,920 12 View

Can anyone suggest a reason for incorrect results during my PCR product transformation?

I have been trying to do a site directed mutagenesis on a plasmid.I have been using the protocol for the PfuUltra II fusion HS DNA polymerase. From the gel it looks like the PCR as a success with...

07 August 2014 3,759 12 View

I have a steady state kinetic reaction. How do I fit the values of the velocities to get the Vmax and rate Km's for the substrate?

I have a steady state reaction of an enzyme involving 2 substrates. I have the velocities and the 2 substrate concentrations. I need to know the type of the reaction that is catalyzed, i.e....

05 June 2013 3,559 5 View

What does negative readings in spectrophotometer denote. Was testing TSS content of bacterial cultures?

I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...

22 July 2024 9,576 4 View

My article: The Extended Pedersen hypothesis can you correct the the name of first Author?

In My article: The Extended Pedersen hypothesis (published, February 1988 Clinical Physiology and Biochemistry 6(2):68-73,) there is mistake of the name of first Author: it is not Catherine...

11 July 2024 1,801 2 View

Is it possible to generate a (semi-)permanent "transcription bubble" within a replicable plasmid?

I was wondering if it is possible to form a permanent open "ssDNA bubble" similar to a transcription bubble (>13 nucleotides) within E. coli. These criteria are important: 1. Open ssDNA...

07 July 2024 8,275 5 View

How to dilute essential oil from plants to assess the biological activity?

I have extracted essential oil from a medicinal plant and want to make several concentrations for some biological activities (Antidiabetic assay). How can i prepare the several concentrations to...

04 July 2024 8,100 4 View

Nutrition map: What does it mean for you?

Unluckily, just similar to an expensive car, your brain can be damaged if you ingest anything other than premium fuel.

23 June 2024 2,287 7 View

Have pathomorphological studies of the lungs been performed in patients with desminopathy?

It is known that patients with desminopathy often die from pneumonia. Have pathomorphological studies of the lungs been performed in patients with desminopathy?

15 June 2024 1,355 7 View

Luciferase assay lysis?

Hi! I'm doing a renilla luciferase assay with coelenterazine and HEK293T cells transfected with AP-1 renilla luciferase. I'm wondering if the cells need to be lysed prior to adding coelenterazine...

13 June 2024 9,936 5 View

How to prepare 0.28 U/ml working solution of Acetylcholinesterase inhibition assay for anti-Alzheimer activity?

I have purchased Acetylcholiesterase from Electrophus electricus (electric eel), C3389-2KU, following details are written on the enzyme vial. Type-VI S lyophilized powder 200-1000 Units/mg...

31 May 2024 6,444 1 View

What is the difference between absorption and adsorption?

30 May 2024 4,447 27 View

What effect may biochar types produced from grape stems, leaves, onion leaves and stems, asparragus and cantaloupe stems/leaves have on a harvest?

I'm interested in knowing the differences in the effects caused by four different types of biochar: cantaloupe leaves/stems, onion leaves/stems, grape peels/stems/leaves and asparragus...

28 May 2024 4,907 0 View