Esha Sehanobish

4 Questions 9 Answers 0 Followers

Questions related from Esha Sehanobish

Does anyone have experience of using thrombin to cleave off the His tag and some adjoining amino acids from a protein?

How does one obtain the required protein by separating out thrombin from it?

11 November 2014 8,889 12 View

Can anyone suggest a reason for incorrect results during my PCR product transformation?

I have been trying to do a site directed mutagenesis on a plasmid.I have been using the protocol for the PfuUltra II fusion HS DNA polymerase. From the gel it looks like the PCR as a success with...

08 August 2014 3,729 12 View

I have a steady state kinetic reaction. How do I fit the values of the velocities to get the Vmax and rate Km's for the substrate?

I have a steady state reaction of an enzyme involving 2 substrates. I have the velocities and the 2 substrate concentrations. I need to know the type of the reaction that is catalyzed, i.e....

06 June 2013 3,536 5 View

Do we have examples of oxidases that do not follow the ping pong mechanism when 2 substrates are present?

Generally most oxidases follow the ping pong mechanism, when 2 sustrates are present. Is there an exception to this rule?

05 May 2013 2,189 2 View