Hi everybody, I want to know if someone of you use this antibody and in which conditions (washing and blocking, diluitions, ecc...) for good WB assay!
Hello, Do you have experience with this type of melting curve? Can anyone explain to me why there is a lowering of the curve? I'm using SYbr select master mix on quant studio 5 real time pcr.
21 May 2024 1,191 0 View
I'm having a problem with SDS-gel. I cannot get the protein bands to run fully on the gel. For me it's the first time with SDS-gel. I prepared a polyacrylamide gel with 5% stackyng gel and 15%...
10 March 2024 228 2 View
According to enzymatic method for extracting extracellular DNA in biofilm matrix, is suggested to use CTAB solution. Why? I only need extracellular DNA... Do you have a similar protocol to...
20 February 2023 2,297 1 View
Hello everyone! I am looking for papers having as topic/subject what follows: 1. Immigrant adults that learn italian as a second language in Italy 2. Adults who study italian as a foreign...
03 February 2023 8,195 4 View
dear all, I am looking for a bit of advice about solubility of fluorescent lipids in oil (DEP or mineral oil). my protocol to create an oil+lipids mixture says (at least) 5 cycles of heating...
29 September 2022 4,596 0 View
Good evenining, I have panel data with fixed effects and my variable of interest is standardised. My outcome variable is binary (decision to implement a trade policy). My coefficient is: 0.019....
28 August 2022 7,946 1 View
Is there anybody who has interesting articles about Awake Surgery in bilingual/multilingual patients? Maybe some comparisons with monolingual patients undergoing the same procedure. Thank you!
09 January 2022 2,776 7 View
Hello everybody I need a piece of advice on this topic: I am working with oil in water droplets decorated with a fluorescent tag and ssDNA To avoid droplets' coalescence I need to add a...
10 October 2021 6,002 3 View
Hi, I'm looking for some scientific literature regarding the role of SLP in voice changing for transgender patients. Could anybody help me? Thank you!
15 September 2021 2,488 0 View
Hi everyone, I'm looking for some articles about how scoliosis can affect swallowing leading to dysphagia. Can anyone help me? Thank you! Alessia
13 September 2021 326 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...
31 July 2024 4,510 3 View
I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...
23 July 2024 6,664 6 View
Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....
23 July 2024 7,292 3 View
I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...
22 July 2024 9,160 2 View
Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...
21 July 2024 1,044 1 View
when we grow biofilm in test tube, the pipette often do not reach near the content. so we have to decane it directly or use a smaller test tube.
17 July 2024 3,532 0 View