Alternatives to traditional cloning of insert into backbone (dsDNA)?

Hi, I am stuck with a cloning experiment: The insert & backbone(bb) share one compatible end at 3' end(NotI) and one incompatible end at 5' end (XbaI for insert; HindIII for backbone). So i...

16 February 2021 1,664 3 View

How structure of monomers in molecular imprinted polymers influence their selectivity?

Hello, I'm biologist and currently working on MIP. I wanted to know is there any literature that mention the influence of monomer structure (for example size of molecule) on MIP selectivity and...

27 October 2020 1,571 6 View

What mask can be used for ultra-deep KOH etching on 110 Si?

Hello everyone! I need to etch Si to the depth of 1mm approximately, not affecting the surface of the sample (which is atomically flat and must be kept as it is, so we can't anneal the sample)....

16 August 2020 9,727 1 View

Are there any papers where advantages and disadvantages of different drug delivery systems are observed?

I'm writing PhD thesis and need to compare different drug delivery systems applied to SOD delivery.

22 July 2020 7,210 2 View

Which molecular structures are stained by trypan blue and ink-vinegar for visualization of mycorrhiza?

What do these substances stain exactly?

20 June 2020 8,653 1 View

How to calculate the band structure for a two-dimensional triangular lattice?

You can provide links with calculation and clear examples of the methods used. I would also like to understand how to take into account a specific potential. How to determine the forbidden zone...

09 June 2020 9,728 2 View

Can anyone advice empirical papers on ideas and their impact on institutional change?

Does anyone know any good papers on the impact of the political elite's ideas on institutional transformations in terms of comparative politics? It will be really great if there are both...

27 February 2020 3,665 3 View

Could someone recommend a journal similar to Biodiversity Data Journal?

I'd like to publish some ideas about taxonomical database. This is possible to do in Biodiversity Data Journal or PeerJ, but I need to pay for an article. Does anybody know similar journals...

23 February 2020 6,876 3 View

Is "green energy" really "green"?

Is "green energy" really "green"? Is Green Energy Really Eco-Friendly? Isn't solar panel manufacturing too dirty? Is it not too damaging to put wind turbines in farmland? Don't we pay too much...

24 January 2020 4,067 14 View

Is it possible to purify the protein using Ammonium Sulfate precipitation only?

Dear All, I am about to start purification of many variants of not tagged recombinant protein expressed in E.coli. I need it to be at least 70% pure. Column purification would be very time...

09 January 2020 7,116 4 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

How to find if Qualitative and Quantitative (discrete) variables have a relation?

I'm going to do a brief research about the dependance between the number of cases of Covid-19 and sex( male/female) . One of the requierements is to use inferential statistics to test the...

18 February 2021 7,092 11 View

Why does the testosterone dissolved in dmso and methanol turns into a white dispersed precipitate when subjected to serum free media?

1mg of testosterone is dissolved in 20 microlitre of DMSO and Methanol respectively. The interpretation showed a positive result. However, when 1 ml of serum free media is subjected to the...

27 January 2021 8,213 1 View

Is there a formula or standard protocol for steroid hormones treatment in mice or rats?

hi, I'm going to determine the dosage of testosterone in order to inject to rats or mice subcutaneously. I wonder that is there a particular dose or multiple doses for that or not. thanks.

05 January 2021 5,921 2 View

Hi! What is the best way to define/specify content-related factors and style-related factors in terms of language variation / gender differences?

28 December 2020 4,409 3 View

Is there any website where I can find daily COVID-19 confirmed cases and deaths according to age and sex for Sri Lanka?

I need to calculate CFR based on age group and sex. For this, I need daily COVID-19 confirmed cases and deaths according to age and sex for Sri Lanka. Where can I find this?

13 December 2020 8,287 2 View

Can the potential of LIPIDMICS improve the diagnosis and understanding of metabolic changes in various neurodegenerative disease (PD, HD, AD, MS, etc?

08 November 2020 7,209 1 View

Does anyone know of any scales/measures/questionnaires that are available to measure stress and well-being?

My research is on the relationship between unemployment and stress and well-being, and how gender differences moderate that relationship. I'm quite new to research, as this is for a university...

13 October 2020 4,096 13 View

How do I test for gender differences in Spearman rho correlations?

I want to explore if there is a gender difference in a correlation between two ordinal items. I have calculated spearman rho within gender for the items, but I now need to test if the correlation...

09 October 2020 970 13 View

What is the most sensitive Non-invasive prenatal testing for fetal sex determination?

Is 8th week the earliest week where we can determine the fetal sex ?

05 October 2020 9,576 9 View