What is the difference between a shared aperture antenna and a dual band antenna?

Can the dual-band antenna be called as a shared aperture antenna? Difference between shared aperture antennas and dual-band antennas?

30 December 2020 7,118 4 View

How to make a standard curve for qpcr?

Hi there, I am working on airborne microbes. I want to know the concentration of bacteria in my sample before I go for NGS. Since it is an environmental sample it might have a lot of mixed...

17 November 2020 314 3 View

How to cluster the ORF sequences?

Hi, I have found the ORF region of my nucleotide sequence which was obtained from Sanger sequencing using 16s universal primer. I have to cluster them before using them in my model. I am using...

13 August 2020 1,079 4 View

ARIMA with Sliding window?

I have 245 daily stock returns with some explanatory variables. I will compare the ARIMA and ARIMAX models with some basic machine learning techniques. The training periods will be 30, 60, 90, 120...

09 August 2020 9,344 1 View

Difference between point source, line source and plane wave excitation in FDTD?

Excitation in FDTD could be point source, line source and plane wave. What is the difference between the three? Also which type of source could be used for various applications like antenna,...

08 May 2019 4,691 4 View

How to display a 3 Dimensional matrix by their values in Matlab?For example if x is a matrix of size 20x15x6.How to visualize the structure in MATLAB?

For example to model a microstrip patch using FDTD. The 3D array would be of size MxNXP. How to pictorically represent the structure using MATLAB?

08 January 2019 6,765 4 View

What are the alternatives to TexGen, textile geometry modelling software?

I have been using TexGen for sometime now and I need to create layers of textile in different orientations and I haven't found a way to do it in texgen. Are there other softwares that are good for...

24 May 2018 9,506 4 View

How can I draw dot plot (Column scatter graphs) for different groups and denote comparisons?

How to draw dot plot for different groups and denote comparisons ? (See attached images) If there is a procedure in SPSS/ Excel or if there is any free user friendly online S/W guide me. Thank you

22 June 2016 7,644 8 View

Where can I find Vernonia oil (seed oil)?

21 May 2016 6,230 2 View

What is the best (updated) book for molecular biology (Cloning)?

03 April 2016 4,207 7 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

What is this fern?

Please help me to identify this fern species. Thank you in advance.

24 February 2021 2,435 5 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View