Do neurons durotax?

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During chemotaxis boyden chamber assay, I see cells migrated preferentially at the insert edge. What is the reason for this?

After 3 hours of incubation of cancer cells (in 150ul media 1%BSA) on top of the 8um pore size insert with known concentration of chemo-attractant at the bottom(1ml of media+chemoattractant...

08 September 2018 1,613 3 View

Is there a way to prevent agarose drop from floating after addition of media?

Hello everyone, My experiments require me to form a 0.5 wt% agarose with a certain chemokine on a 24 well plate. This would allow slower release of the chemokine and we want to check the...

07 August 2018 10,065 1 View

Is there a paper that correlates effect of good,partial and poor solvents on PCL viscosity?

1) It would be really helpful to get good paper that explains how solvent type effects PCL viscosity. 2)Also, a good paper that explains the technique/procedure used to measure the viscosity...

11 December 2016 1,527 4 View

What is the preferred method of finding the wettebilty of a single microfiber?

I wish to peform a contact angle measurement on a single PCL fiber with diameters ranging from 2 to 10 micrometers. Is there any publications that have used such methods/instruments? Thanks

11 December 2016 5,237 5 View

Can anyone please provide me with a proper step to step guidance in measuring the viscosity of PCL solution using a rheometer?

A paper/link to give a brief guidance on how to measure the viscosity using rheometer so that I can tackle questions like" 1)the geometry to be used 2)which test to be performed? 3)range of...

11 December 2016 9,108 4 View

Which surface tension measurement technique is best for a PCL solution with chloroform as solvent?

A quick approach to measure the surface tension of PCL and Chloroform blend is required since chloroform is highly volatile.

10 November 2016 3,159 5 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View