During chemotaxis boyden chamber assay, I see cells migrated preferentially at the insert edge. What is the reason for this?

After 3 hours of incubation of cancer cells (in 150ul media 1%BSA) on top of the 8um pore size insert with known concentration of chemo-attractant at the bottom(1ml of media+chemoattractant...

08 September 2018 1,494 3 View

Is there a way to prevent agarose drop from floating after addition of media?

Hello everyone, My experiments require me to form a 0.5 wt% agarose with a certain chemokine on a 24 well plate. This would allow slower release of the chemokine and we want to check the...

07 August 2018 9,939 1 View

What is the preferred method of finding the wettebilty of a single microfiber?

I wish to peform a contact angle measurement on a single PCL fiber with diameters ranging from 2 to 10 micrometers. Is there any publications that have used such methods/instruments? Thanks

11 December 2016 5,098 5 View

Is there a paper that correlates effect of good,partial and poor solvents on PCL viscosity?

11 December 2016 1,419 4 View

Can anyone please provide me with a proper step to step guidance in measuring the viscosity of PCL solution using a rheometer?

A paper/link to give a brief guidance on how to measure the viscosity using rheometer so that I can tackle questions like" 1)the geometry to be used 2)which test to be performed? 3)range of...

11 December 2016 8,998 4 View

Which surface tension measurement technique is best for a PCL solution with chloroform as solvent?

A quick approach to measure the surface tension of PCL and Chloroform blend is required since chloroform is highly volatile.

10 November 2016 3,058 5 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

No title

02 March 2021 3,060 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View