Hi every one, I have questions about neutrophil adherence.

I isolated neutrophils using Polymorphprep.

After isolation, I seeded it to 12-well plates with RPMI 1640 medium (+10%FBS, 1%PBS) and stimulated it with LPS.

Methodologically, it is supposed to attach to the culture plate after activation.

However, I found that both unactivated and activated neutrophils not strictly attach to the plate at all, it's almost all suspension state.

When I check the inflammatory cytokine with the qPCR, it seems that the neutrophils were activated successfully.

But I wonder is it not normal for this unadherent neutrophils?

Do anyone have experiences with this, please share with me.

Thank you so much.