Do Fagus sylvatica (European beech) and/or Picea abies (Norway spruce) develop dimorphic root systems?

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When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

What is the best temperature to coat an ELISA plate?

Hello, I want to control the temperature of my ELISA plate during incubations to get consistent results. In general, what is the best temperature to coat the plate with antibody? I have been...

16 February 2021 2,377 7 View

SPSS - Merging files by ID numbers that contain letters?

Hi, I'm trying to merge files from a data base of 20 files, each containing about 4000 subjects. Unfortunately the ID numbers are actually a combination of numbers and letters (e.g., b1458219)....

15 February 2021 5,833 3 View

How would I go about getting an expert review on the interview questions for the dissertation?

Good Evening, My name is David Marshall and I am Doctor of Management Student at Colorado Technical University Online. I just recently received approval from the IRB to start data collection on...

08 February 2021 346 7 View

How can I quantify the color of a solution?

I know colorimetry has been the standard way by way of measuring the concentration of a particular compound (solute) in a colored solution (solvent or measure quantities of a particular compound...

08 February 2021 2,673 1 View

ACE2 Blotted as a double band?

Hello, I have recently run a western of Vero cells for ACE2. I detected ACE2 at the correct size, but it presents as a double band. The sample was prepared by lysing cells in 1X Laemmli...

07 February 2021 6,973 3 View

SARS-CoV-2 Spike Protein Western Bands?

Hello, I am western blotting SARS-CoV-2 spike protein from lentiviral using an antibody against S1. To prepare samples, I mixed concentrated lentivirus 1:1 with 2X Laemmli buffer and boiled...

07 February 2021 3,643 4 View

How does DMSO percentage affect ELISA?

Hello, In my sandwich-type ELISA which is testing for small molecule inhibition of a protein-protein interaction, it seems that DMSO percentage and dilution protocol are important. I...

31 January 2021 6,771 1 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

Are cloud points able to identify tree species?

Hello Dears My goal is to classify dense forest tree species using drone images. Is it possible to classify dense cloud points? If yes, in what way and in what software? Thankful

15 February 2021 3,475 3 View

What is the correct common name for Hydrochoerus hydrochaeris?

There are two extant species of Hydrochoerus: Hydrochoerus isthmius, the lesser or Panamanian capybara, and the genotype species H. hydrochaeris. The latter is the more common species of capybara,...

11 February 2021 3,494 14 View

Loss of biodiversity its impact on terrestrial ecosystem?

I need research papers with problem statement as well as research questions in respect of the above mentioned topics

04 February 2021 2,240 15 View

Is there any procedure or an equation for converting total carbon to organic carbon or any equation that helps to obtain the value?

I have analysed percentage carbon (total) using CHNS Elemental Analyzer for the forest soils. Now, I would like to obtain SOC content from the obtained value for which I am looking for a procedure...

29 January 2021 5,551 8 View

How to plot Random Forest model as raster prediction map in RStudio?

Output_raster

27 January 2021 7,974 3 View

What is the magnitude of yearly rhizopdeposition in tropical forests?

Hi all, I am trying to find reference values on annual soil rhizodeposition rates for different kinds of forests, with a particular interest for tropical rainforests. Do we have an idea of the...

26 January 2021 4,278 4 View

Do you have any information on modeling the diameter growth of conifers?

Hello dear friends Regarding modeling the growth diameter and cross section of conifers, I need a number of articles that have researched in this field. have you any recommendation? Thank you for...

20 January 2021 6,532 4 View

How the microplastics levels in freshwater and marine water ecosystem be controlled?

Microplastics have been found in land, air, freshwater, effluent from a wastewater treatment plant and even in tap water and bottled water. The small plastic particles (less than 5 mm) pose a...

19 January 2021 8,637 3 View

What is the minimum number of plots to apply i-Tree Eco assessment for carbon storage and sequestration on a 700 hectares of urban forest ?

I want to do a sample inventory of a 700 ha forest to have the amount of biomass and apply i-Tree Eco to determine carbon storage and sequestration. I would like to know the minimum number of...

17 January 2021 5,343 3 View