Hi everyone,

We have isolated total RNA from our samples using Qiagen RNeasy Plus Micro Kit (which includes genomic DNA removal columns), obtaining RNA yields of around 100 ng. These samples are going to be used for RNA sequencing, with the NEBNext Ultra II Directional kit and NEBNext Poly(A) mRNA Magnetic Isolation Module. Has anyone treated the RNA samples with DNase before proceeding to the library preparation? As we are worried with the risk of losing some of the RNA yield that we have, we would appreciate any recommendation about which would be the best way to collect back the RNA after the DNase treatment (magnetic beads or clean-up kits?), so if someone could provide us with a protocol for this step, it would be very helpful.

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