DNA isolation from jellyfish

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Can other standards be used to generate IRF file instead of LaB6,Si etc.,,, for Rietveld Refinement?

Hi, my research involves in ZnO synthesis. I have performed rietveld refinement of my samples and obtain a good fit using Fullprof suite software. However to obtain the crystallite size, the IRF...

07 July 2024 3,526 3 View

How to calculate corrosion rate from potentiodynamic polarization test data for dissimilar welds?

we need surface area, corrosion current, equivalent weight and density of the metal involved for corrosion rate calculation in mm/year (lets say). For dissimilar metal welds, how can we measure...

30 June 2024 5,943 2 View

How to convert energy count spectrum into 2D/3D image?

Respected researcher, I want to know that is there any way or one can help in generating a 2D/3D image using the energy count spectrum of from backscatter methods. How can I design such an...

29 June 2024 10,023 1 View

What is Academic Research Paper?

I do a research about Non-Verbal Communication: Gestures and Body Language in Classroom.

18 June 2024 8,737 1 View

Can i deduce the presence of surface states based on my XPS data?

In my XPS spectral, i detected oxygen group bonded to carbon and in my zinc spectral (note : my sample is ZnO) ...there are two valent zinc species available, (which is unsual as my previous...

17 June 2024 3,888 2 View

Action research design is Quantitative Studies or Qualitative Studies?

14 June 2024 1,684 1 View

How should I interpret this moderation analysis (PROCESS Macro)?

Hi there! I was wondering how I should go about interpreting the following data. I am currently investigating the moderating role of working memory (continuous) on the relationship between...

07 June 2024 1,473 0 View

I need help?

Is there anyone to tell me the scientific name of this mushroom?

03 June 2024 464 5 View

How Through Silicon Vias (TSV) simulated in Silvaco?

How can we simulate TSV in the Silvaco example? Exactly, I need the instructions for the attachments file. Best regards.

29 May 2024 1,638 1 View

Best firm level database for south Asian firms?

best firm level database for south Asian firms?

28 May 2024 3,424 0 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

How to quantify polystyrene microplastic (8 micron) bioaccumulation in fish tissue?

"I have treated adult zebrafish with 8-micron polystyrene microplastic and want to study the bioaccumulation in different organs. Can this be done using hydrogen peroxide digestion followed by...

05 August 2024 853 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Is it common for heterotrophic bacteria to grow on Pro99 and SNAX media?

I am attempting to isolate picocyanobacteria from seawater (pre-filtered with a GF/D membrane) using the pour plate technique and filter plating method (Kearney et al., 2022) on Pro99 and SNAX...

30 July 2024 1,040 1 View