I need to know the range for DNA and RNA quantification in NANODROP and EPOCH Microvolume spectrophotometer. Is 2ng/ul a reliable measure?
The as the solution were mixed together, there was no color change, and then the UV-vis was read every 10min but barely any absorbance was observed. The solutions were 2,1mM of NaBH4 and 0,07g...
20 May 2024 615 1 View
How can the in vitro cytotoxicity data of an extract be extrapolated for evaluation in an in vivo tumor model?
08 August 2022 3,510 4 View
Hi! I am working with cell suspention cultures (different lines of arabidopsis and nicotiana) , I have a linear shaker and I need to know if someone has worked with this because protocols...
08 August 2022 9,262 1 View
Hi everyone! I found a very interested course in YouTube, that mention this link to get the info and more related materials: https://www.csc.fi/web/training/-/scrnaseq but the link is not working...
23 March 2020 9,057 3 View
I have a doubt of compatibility between the cap strip form Thermo Scientific and Bio-Rad plates for real time PCR. I know that Opticon thermocycler read fluorescence from the top of the plates, I...
05 October 2014 8,468 3 View
I want to do an immunolocalization of two protein in an embryo. They localize in the same type of cells. What do you think about double immuno? Is it better to coincubate or not? This question is...
21 May 2013 3,074 2 View
I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.
11 August 2024 9,101 4 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...
10 August 2024 7,180 0 View
I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...
10 August 2024 7,429 2 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View