what happens if with the DNA purity value as I attached in the picture, and I still force it for the amplification process? does RNA contamination interfere with the process
What are you trying to do, here?
Spectrophotometry does not distinguish between RNA and DNA: nucleic acids absorb at 260, and that's just it.
So...you might have RNA, might have DNA, might have both. You can't tell.
If, for example, you isolated DNA using a DNA isolation kit (with RNAse A), then...no, you don't have RNA.
If, in fact, you isolated DNA from anything other than the purest, most carefully maintained sample ever, using chaotropic salts at all points, then probably...you don't have RNA.
RNA isn't the easiest thing to isolate intact even when that's exactly what you want to do, and it's even harder to isolate by accident.
BUT
Let's assume you have RNA contamination, but want to use your DNA for PCR.
...Use your DNA for PCR. Just..ignore the RNA. It won't be a productive template for your reaction, it won't be a stable template for your reaction, and it can more or less be safely ignored.
It would, in fact, be lovely if RNA could be used directly as a template for PCR, but it can't. So, don't worry about it.
If you want to use your DNA for WGS or other next-gen sequencing approaches, they usually prefer you to remove RNA primarily because it gets in the way, chelates adaptors during ligation, etc. You can do this by a simple RNAse treatment: getting rid of RNA is really easy (keeping it is harder).
A more prominent issue here is that you have 260/230 ratios of ~0.6, which indicates fairly significant guanidium contamination (usually): this can interfere with almost everything, including PCR.
This is more of a problem for RNA (where you need to make cDNA, using fairly large amounts of RNA) than for PCR (where you can use tiny amounts of template DNA), but still.
So, again: what are you trying to do, here?
Greetings
As the enzymes of the process of DNA AND RNA amplification are different, you won't amplify RNA instead of DNA.
Another point is that RNA is not steady enough and will degrade easily.
But the contamination will make it difficult for Enzyme to find its substrates
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