For a long time we used a Yellow Spring instrument Co - YSI 5300 biological oxygen monitor that allowed us to measure mitochondrial respiration and things like that. Those electrodes were very sturdy, though a bit noisy at times and has finally died.
Does anyone have experience in O2 electrodes for biological applications that are more affordable than the Oroboros? I am particularly interested in instruments with robust electrodes...
thanks!
Matias
Dear Sir. Concerning your issue about the dissolved oxygen electrode for biological applications. Air can be regarded as having a constant percentage (approximately 20.9%) of oxygen. Wherever air comes in contact with water, the oxygen in the air will dissolve in the water. The amount of oxygen that dissolves in the water depends on many factors: whether there is adequate time and adequate mixing to fully saturate the water, the water temperature, the air pressure, the salt content of the water, and whether there are substances in the water which consume the oxygen. Since the oxygen content is important for many biological and chemical processes, measurement of the amount of oxygen actually dissolved in a water sample is of great importance. Applications for dissolved oxygen measurement include processes where the amount of oxygen affects a reaction rate or process efficiency, or indicates an environmental condition. Some important applications include wastewater treatment, wine production, bio-reactions, and environmental water monitoring. Thermo Scientific Orion DO instruments have the flexibility to use polargraphic and optical technology for DO measurement needs meeting regulatory requirements. Membrane oxygen electrodes are the most widely used and accepted method for real-time measurements of oxygen in water. Learn more about how Thermo Scientific instruments can help you meet your regulatory needs.I think the following below links may help you in your analysis:
https://www.thermofisher.com/sa/en/home/life-science/lab-equipment/ph-electrochemistry/dissolved-oxygen-measurement.html
https://pubs.acs.org/doi/abs/10.1021/ja01278a073
Thanks
Hi Matias,
there are different vendors:
http://www.globalw.com/products/durox.html
https://www.fondriest.com/environmental-measurements/equipment/measuring-water-quality/dissolved-oxygen-sensors-and-methods/
http://www.lazarlab.com/disoxy.htm
https://www.ysi.com/parameters/dissolved-oxygen
https://www.thermofisher.com/de/de/home/life-science/lab-equipment/ph-electrochemistry/dissolved-oxygen-measurement.html
https://www.warneronline.com/product_info.cfm?id=323
https://camblab.info/wp/index.php/what-type-of-electrode-should-i-use-to-measure-dissolved-oxygen/
https://www.vernier.com/manuals/do-bta/
http://www.horiba.com/sg/application/material-property-characterization/water-analysis/water-quality-electrochemistry-instrumentation/support/product-selection-support/technical-tips/electrodes/dissolved-oxygen-electrode-care-and-maintenance-procedures/
http://www.finesse.com/products/sensors/single-use-sensors/phplusdo2-smartpuck/
Regards
Markus
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