Hi! Can you help me regarding this matter, I am going to do an Egg Albumin Denaturation Assay on the formulated ointment with 5g plant extract. The ointment's base is beeswax and olive oil. However, I cannot find a good solvent to dissolved the wax. Please recommend. Thank you
Abdullateef Ajege
Hexane or Heptane:These are non-polar solvents effective for dissolving beeswax. They evaporate quickly, leaving the dissolved material. Ensure you work in a well-ventilated area due to their volatility. Toluene:Effective for dissolving beeswax and compatible with oil-based systems. Use with proper ventilation as it is hazardous if inhaled. Chloroform:Efficient for dissolving both wax and oil. Handle with care in a fume hood. Ethanol (High Concentration, e.g., 95%):Beeswax is partially soluble in ethanol but might require heating for complete dissolution. This solvent is less toxic compared to others. Isopropyl Alcohol:Like ethanol, it might need gentle heating to dissolve beeswax. Its safety profile is suitable for assays. Regiez Novem Idulsa
To dissolve beeswax in preparation for your Egg Albumin Denaturation Assay with the ointment, you can use a non-polar organic solvent that is compatible with your formulation and safe for the assay.
Recommended Solvents:
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