17 January 2020 10 982 Report

Many microbiology processes involve:

1. Growing a starter culture to high density

2. Back diluting into fresh media by some dilution factor, eg, 1:100

3. Growing to OD X (eg, OD=0.5)

4. Harvesting for some assay, extraction, or process

My question is: How can you predict/understand the dilution factor necessary?

In this case, I'm asking about RNA extraction for qPCR, but I'm interested in the question more broadly.

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