how would autoclaving and addition of optimum dosage of sodium azide affect the activity of the microbes in the sludge besides the EPS released?
Autoclaving kill almost all the bacteria while sodium azide mostly only inhibit metabolism. Much of the bioactivity in sludge can resume if sludge is washed and reconstituted in fresh water after preservation by azide.
https://en.wikipedia.org/wiki/Sodium_azide#Biochemistry_and_biomedical_uses
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