I have just begun to cultivate M.methanica 25384 as pure culture. I transferred the culture ordered from DSMZ into the medium 1313 as suggested. The strain grew differently among the triplicates, but it grew. Then I used the most performing culture as inoculum for the "second generation" of M.methanica, but it did not grow.

Did someone get the same problem? Any idea of what is going on?

I have already planned to re-inoculate from frozen original stock to check if the problem is the culture propagation.

Thanks

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