Did everyone incubate adult zebrafish with virus in media?

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Is it safe to make a glycerol stock from Agrobacterium transformed cells from a 10-day plate?

Hello all. I transformed some Agrobacterium competent cells by electroporation and performed colony PCR to see the positive transformants. However, I performed the glycerol stock after 10 days of...

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Is it safe to purify plasmids of E.coli precultures from 2-month old plates?

Hello all. I purified some plasmids after doing precultures of strains from a 2-month old plate in -4 degrees. The PCR results were fine and I used theses plasmids to transform Agrobacterium...

26 April 2024 8,440 3 View

How has the efficiency of photovoltaic cells improved over time?

On the use of solar energy in homes.

07 April 2024 9,465 2 View

Can someone indicates me a database about meteorological variables per country?

I'm looking for variables like solar irradiation, wind speed, humidity, temperature and precipitation

06 April 2024 1,926 2 View

How to prepare dibenzo[a,h]anthracene stock solution? Which solvent to use?

I have trouble solubilizing the analytical standard of dibenzo[a,h]anthracene (a polycyclic aromatic hydrocarbon purchased from Sigma-Aldrich) to prepare a stock solution. I have already tried...

01 April 2024 5,768 3 View

Mitosox in mitochondrial extract?

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24 March 2024 466 0 View

How can I filter kraft lignin?

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Which stain can be used for visualizing qunencher-tagged DNA oligonucleotide using gel electrophoresis?

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What the best protocol for protein extraction from human neutrophils?

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Does anybody know how to make homemade ABI 3500 buffer ?

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Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

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Weak DAPI staining after immunohistochemistry - how to improve?

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The Curse of Evolution and Complexity?

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For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

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How to isolate lymphocytes from mouse spleen?

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How to start a Molecular Dynamics Simulation?

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Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

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Sijie Lin

when it comes to virus, I don't think exposure through media is recommended. It is difficult to control the amount of virus exposure and it is a health risk to do so. microinjection is always recommended and it gives you the precise control of the amount of virus introduced to zebrafish.

Aletheia Lima Souza

Thank you very much for your reply Sijie Lin.... I wil adjust my protocol

cheers

Hanadi Abdulelah Abdulrazzaq

It is difficult to control the amount of virus exposure and it is a health risk to do so.