I need to extract total RNA from whole blood samples in order to perform RT-PCR. I tried PureLink® RNA Mini Kit, but RNA yield was low and quality was bad. Blood samples were fresh and stored in EDTA tubes. I tried to extend lysis time but the results were same. Does anyone have suggestions about this problem?
Dear Mohammad Pourhassan-Moghaddam,
Thank you for your answer. I extracted RNA from whole blood and I already used column-based method. I think problem is the initial blood sample volume (200 ul). But kit works with up to 200 ul pf fresh or frozen blood. I don't know how I yield high RNA with this kit?
Dear Zeynep,
We have used this kit in our lab once. The kit worked perfectly from the first time itself (though, I have used it for fibroblast cell lines). Please take proper care during the wash steps and perform sequential elutions for obtaining yield of RNA (sometimes, the RNA is left behind in the membrane, if not eluted sufficient times).
Hope, the issue gets resolved.
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Biology
- Molecular Biological Techniques