specially in roses.the protocols and the lab work Did anybody experience micrografting technique of plants with in Vitro culture?
I'm interesting in micrografting in fruit trees and looking forwd to share my experinces with you ...my e-mail: [email protected]
Micrografting is commonly used in watermelons, tomato and easy in solanaceous species. I don't think there are any published protocols for rose. I have attached our paper on grape micrografting for virus indexing.
Dear Mahboubeh
Please see attached file as your question response.
Regards
Dear Coworkers,
Thanks alot for your answers. My exact problem is that the stem diameter of the native roses in vitro condition is so narrow and weak. Do u have any suggestions to increase their growth except the application of Amino acids (like Casein or Galantamin) and AgNo3?
You may exceed the duration of culture. For example 10 weeks for the rootstock and 6 weeks for the scion. Also you may increase the amount of cytokinin and light intensity for more compact plants.
Stem diameter is genetically controlled and you say you use native roses. If they are climbing types, the stem diameter will be smaller than bushy roses. Nevertheless, if you cut the scion at a sharp angle, you should be able to insert it to rootstock.
Hi I have used micrografting technique using transgenic invitro apple plantlets onto apple rootstocks to prepare them for inoculation. The success rate was very high upto 98%. If you want more details, I can provide.
You can review the research done in Avocado (Persea americana) by Richard Litz, Simon Riharjo and Witjaksono.
I have an experience with in vitro grafting of grapes. I am using different rootstocks and varieties. The graft union rate is between 80-90 % with cleft grafting method. If you need more information I can help.
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