Dialysis membrane for protein purification?

More Manoj Kumar's questions See All

Peaks in FTIR analysis?

I have analysed 2 protein extracts from same source. The peak of one extract (A) is more sharp then other (B). Can we say (A) is more pure than (B)?

01 February 2019 9,035 4 View

Dialysis bags for purification of protein?

I am extracting protein from cottonseed meal and I have to purify around 500 ml protein solution using dialysis membrane. I am looking for large volume dialysis bag which can hold 500 ml of crude...

31 December 2018 1,244 10 View

What is the reason for current dropping in OER , LSV curve?

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10 August 2024 3,629 1 View

What may be the reasons for failures of Tube toi Tube Sheet Joints in Boiler Drum ?

We have observed that tube to tube sheet joint leaked in our boiler and needs to overcome same by knowing the root cause.

08 August 2024 3,161 0 View

I want to remove Urea from my protein sample?

I want to remove Urea from my protein (6kDa) sample. I did use : 1. Step down the Urea gradient (dialysis) in order to remove urea and refold my protein, but my protein still contains urea in the...

06 August 2024 1,350 4 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

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What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

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What is the best blank for nanodrop if I want to read a recombinant protein concentration?

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Transfection in HEK293T cells?

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Adam B Shapiro Popular answer

Dialysis tubing can become clogged or fouled. It is also delicate and can break. I would not recommend reusing it.

Adam B Shapiro

Manoj Kumar

Dear Adam, Thank you for your suggestion.

Alaa Jabbar Al-Manhel

Hi Manoj Kumar

Yes of course, you can reusing dialysis membrane for same protein sample by activation (with acetic acid ,EDTA ,hot water .....) but not more than 3 time.

regard