For example i am working on estimation of reducing sugars with the help of DNS
What will be the role of DNS in the reaction and how the colour of DNS changes from yellow to orange colour after we complete boiling water bath
wen 3amino 5 nitro salicylic acid acts on reducing sugars 3nitro group is reduced to amino group (NH2) after this what happens to reduced amino group and how the colour is changed from yellow to orange after boiling water bath reaction is completed