How to measure fluorescence intensity of Dapi stained nuclei?

I have tried various methods using imageJ to measure mean fluorescence intensity of dapi stained nuclei but none of them seem to be working. Ive attached an image i am trying to measure the...

15 February 2021 8,637 3 View

How stiffness matrix modification after hinge formation is done for pushover analysis?

Can anyone please explain this? How do I account for plastic hinges in my model and how do I modify the stiffness after plastic hinge is formed? Need a small example. Thank You

15 November 2020 1,967 3 View

How to model hysteresis property of concrete in abaqus?

I want to model rc element subjected to cyclic loading, How do I enter hysteresis data in the material model? I want to use Mander's 1988 model to get data for concrete. I dont know how to enter...

06 September 2020 7,651 3 View

How to select appropriate value of dispersion coefficient for HecRas 1-D steady water quality model?

I am working on a water quality model for the Mula Mutha river. I have a doubt regarding the dispersion coefficient to be entered. whether to enter a fixed value or computed value? what are...

22 May 2020 848 1 View

Why the ZDOCK output file (.pdb) is not compatible for Chimera?

Why the final docked complex file output from ZDOCK is not compatible for Chimera?

24 April 2020 3,347 3 View

Got frequency of human recorded audio file in the range of 0-8000 Hz. Is it correct range?as per many sources human voice frequency is below 260 Hz?

I tried finding frequency by various tools such as audacity (got frequency 0-16000 Hz). Tried with Librosa(Python package) and got the frequency of human recorded audio files in the range of...

06 April 2020 7,607 6 View

Is there any software which take input as pdf(research papers) and generate the data from all the plots and graph inside it?(Similar to NLP)?

I am looking for software that will take input as a research paper (pdf format) and generate the data from plots and graph in it. as similar to NLP softwares.

04 February 2020 4,244 3 View

How to increase RNA yield and purity from PBMCs stored in RNA later at -80 C?

I have been using miRNA Isolation mini kit (qiagen 217004) for isolating RNA from PBMCs stored in RNA later at -80 C. The cell no. was 2 million cells. The RNA yields obtained are around 30-40...

15 October 2019 4,731 1 View

What is the criteria to select the range of normal stress in Direct shear test?

I did not not find the answer anywhere. Please help.

19 August 2019 9,950 3 View

How can the trigonometric expression be solved for particular variable in MATLAB?

When I try to solve the symbolic trigonometric expression, I am not getting desired results even results exist. The MATLAB code is written as follow: clear all clc syms nq lam alfa ai theta phi...

16 July 2019 1,380 2 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

What are anisotropic electrical and optical properties of a semiconductor due to?

In some materials, properties such as band gap, thermal conductivity differ along different crystallographic axes. What are the basics?

01 March 2021 1,923 3 View

How to define and get results (stresses) for certain points of a model in abaqus, using python? When the size of the model changes?

I want to do a parameter study of a aixsymmetric cylinder in Abaqus. I want to create several jobs where I get the stresses for 3 different points in my model. These points are defined. I wrote a...

01 March 2021 10,078 1 View

How can I integrate a routing protocol in a tiny device (e.g. Raspberry Pi)?

Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...

01 March 2021 9,332 6 View

Joint behaviour in Abaqus is not captured well?

I am working on roof joint and trying to simulate the behaviour in Abaqus. I am successful in running the model but the final graph does not follow the experimental curve in the negative direction...

01 March 2021 6,661 3 View

Which one is easier and better at modeling slender masonry structures? ANSYS or ABAQUS?

I need to model masonry and reinforced concrete minarets for my MSc thesis. Which one is easier and better at modeling slender masonry structures? ANSYS or ABAQUS? (Or any other program for just...

01 March 2021 736 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How to assign different material properties to each element in Abaqus ?

Hello, I am writing a python script to generate my model and write the input file. The problem is I have 1000 elements in my mesh and I want to assign different material properties to each...

01 March 2021 9,511 3 View

How to define tie constraint between rigid body and another part in dynamic explicit model of Abaqus?

Hi dear researchers I connected a rigid body into the part of my structure by tie constraint and then I added displacement to rigid body, in static general step every thing is fine and these two...

28 February 2021 3,442 2 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View