Hello,
Does anyone have access or can provide links to freeware that can calculate the net charge and isoelectric points (@ pH 7.4) of a peptide which contains a non-natural amino acid (ie: does not have a predefined single or triple letter amino acid code)?
AB40 natural sequence: DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVV
The sequence I am trying to calculate is a mutated version of beta-amyloid (1-40), with aspartic acid in position 7 replaced with non-natural amino acid Diaminobutyric acid.
Sequence:
DAEFRH-Diaminobutyric acid-SGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVV
Furthermore, I have a mutant peptide with glutamic acid in position 11 replaced with non-natural amino acid Ornithine.
Sequence:
DAEFRHDSGY-Ornithine-VHHQKLVFFAEDVGSNKGAIIGLMVGGVV
Does this limit me to manual calculation or the use of models? Any help will be greatly appreciated.
Calculate it yourself
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5075173/pdf/13062_2016_Article_159.pdf
Due technical reasons the web server for pI has been moved from http://isoelectric.ovh.org to http://isoelectric.org
Details about Isoelectric Point Calculator and comparison to all other pI algorithms (>15 methods) in Article IPC – Isoelectric Point Calculator
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