Hello. Has anyone ever measured lactic acid amounts using colorimetric methods? I measured lactic acid using this method (Taylor, 1996), but I encountered some issues. Should the control samples, which contain no lactic acid, form a color with the reagent? Additionally, the resulting standard curve was a downward-sloping linear curve; what could be the reasons for these discrepancies?
No experience with the method of Taylor (1996). The classical method of Barker and Summerson (1941; https://doi.org/10.1016/S0021-9258(18)51379-X) seems to provide reliable results
@Antonio De Vega
Thank you for your response. I also used Summerson's method before, but no results were obtained. I think one of the chemicals I'm using has a problem. Do you know if the control sample without lactic acid should produce color with the reagents or remain colorless?
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