Following causes can be excluded:
- too little sample, enzymkojugate, substrate and stop solution
- too little exposure (reaction) time
- wrong handling with the pipette
- too less warm up time after removal from the cooling area
- false photometer settings
- ELISA-Kit used after the expiration date
- any step that is connected with disregarding manufacturer's specifications
The ELISA-Kit was carried out manually.
Enzymconjugate, substrate and stop solution was applied with an electrical pipette, whose battery isn't working well what leads sometimes to problems. So I suppose, that the pipette could be one source of error.
Could you please help me, to find out possible other causes?
I would be gratful for any advice or help, thank you!
Hi Julia
You research FC or SC via ELISA
You obtain same results also with calibrators or only when samples are used, check of the coated monoclonal antibody
If you research FC and low extinction values were seen only for samples, then is possible that extraction buffer not good.
Let me know
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