Kiwifruit (Actinidia chinensis Planchon) is an important specialty fruit crop that suffers from narrow genetic diversity stemming from recent global commercialization and limited cultivar improvement. In a study high-density RAD-seq-based genetic maps using an interspecific F1 cross between Actinidia rufa ‘MT570001’ and A. chinensis ‘Guihai No4’ were obtianed. The A. rufa (maternal) map consists of 2,426 single-nucleotide polymorphism (SNP) markers with a total length of 2,651 cM in 29 linkage groups (LGs) corresponding to the 29 chromosomes. The A. chinensis (paternal) map consists of 4,214 SNP markers over 3,142 cM in 29 LGs. Using these maps, we were able to anchor an additional 440 scaffolds from the kiwifruit draft genome assembly. Kiwifruit is functionally dioecious, which presents unique challenges for breeding and production. Three sex-specific simple sequence repeats (SSR) markers can be used to accurately sex type male and female kiwifruit in breeding programmes. The sex-determination region (SDR) in kiwifruit was narrowed to a 1-Mb subtelomeric region on chromosome 25. Localizing the SDR will expedite the discovery of genes controlling carpel abortion in males and pollen sterility in females.

Please see following link for details:

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4596402/

thanks for kindly answer. Yes I just read article. It is really very useful and We are planning to study A.chinensis. So we can use this study.

many thanks

Please have a look these links and PDF attachment also.

http://www.ishs.org/ishs-article/610_71

https://www.researchgate.net/publication/222134194_Molecular_identification_of_sex_in_Actinidia_deliciosa_var_deliciosa_by_RAPD_markers

https://www.researchgate.net/publication/225649777_Development_of_sex-linked_PCR_markers_for_gender_identification_in_Actinidia

http://link.springer.com/article/10.1007/s00497-012-0191-6

Article Molecular identification of sex in Actinidia deliciosa var. ...

Article Development of sex-linked PCR markers for gender identificat...

Thank you Dr. Arif for comments 

Thanks Mr.Singh. I already read three of the articles but not read one of them. I think RAPD  markers not very useful to determine female hybrids. SCAR markers and some SSR markers more useful than RAPD markers. I already used RAPD markers in my previous study and RAPD didnt work with A.chinensis hybrids. Thank you so much for your close interest and kindly answer.

athe above clarifications are more than enough fot you. Best.