Detection of Reactive Oxygen Species (ROS) in whole bood. What is the best method?

Gabriela De Larrañaga @Gabriela-De-Larranaga

10 April 2023 1 6K Report

I am trying to measure reactive oxygen species in a solution of leukocytes exposed to different antigens such as vaccine excipients or allergens. What do you suggest?

Robert Adolf Brinzer

Either use a colorimetric assay like Amplex Red or look for downstream lipid oxidation products like MDA and 4-HNE.

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