For my project, I use MG63 cell line. These cells grew well in the first passage. But after the second passage, there were many dead cells and the culture medium became turbid and powdery (The culture medium placed in the incubator alone, its turbidity did not increase). Also, the culture medium was cultured on agar to determine bacterial contamination but did not show any contamination. In the next days, the attached cells also detached and died. Also, after changing the culture medium, the orange color of the medium quickly changes to pink within an hour (fresh complete medium contains 10% serum, 1% NEAA, 1% L-glu and 1% pen/strp). What is the cause of this cell death and change in color and turbidity of the culture media? Thankful
First of all it might be due to other types of contamination (e.g. fungi) or expired/contaminated materials. If you are using RPMI-1640 powder make sure you add bicarbonate and keep the solution in the dark. Double check the protocol provided by the manufacturer. If none of these works, here is a reliable protocol for MG63 cell culture you can use instead. Good luck.
https://bio-protocol.org/exchange/minidetail?type=30&id=9453003&utm_source=miniprotocol
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