Saghar Yousefnia

3 Questions 3 Answers 0 Followers

Questions related from Saghar Yousefnia

Despite the fact that the culture medium is not contaminated, what is the reason for cell death and the turbidity of the cell culture medium?

For my project, I use MG63 cell line. These cells grew well in the first passage. But after the second passage, there were many dead cells and the culture medium became turbid and powdery (The...

09 November 2022 3,803 1 View

Has anyone experience about isolation of cancer stem cells from cancer cell lines?

I am trying to isolate breast cancer stem cells from breast cancer cell lines through mammosphere formation. I see perfect spheres under microscope but all of them are CD24+/CD44- and dont show...

18 December 2016 9,785 5 View

Which kind of plates and sizes are suitable for mammosphere formation?

which kind of plates and sizes (6, 24, 96 well) are suitable for mammosphere formation?

08 June 2016 4,582 3 View