Despite repeated effort to clone a 3kb gene in binary vector using conventional approaches, I was failed. Do you have any suggestion for me ?

More Muhammad Jakir Hossain's questions See All

Would you mind Please to see the Question in Description bellow?

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Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

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Did anyone ever try using pLVX-TetOne Plasmid with a copGFP as a selection marker instead of Puromycin?

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Difficulty in cloning of 1kb gene into vector?

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How to use protein A G beads to purify antibody?

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Why are Sanger Sequencing reads interrupted in some edited clones?

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Hi, can anybody help me with the suggestions how can I amplify a stress induced gene of 1107 bp?

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Alternatives to traditional cloning of insert into backbone (dsDNA)?

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Why do I have trouble with cloning duplicate gene in the same plasmid?

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Cyrille Girard

I have no experience with those vectors but IMHO I would use the brute force. It has rescued my clonings many times: use a huge, out of proportion, insane amount of insert relative to your vector...this helps.

Neda Alidadiani

Dear Jakir

your question is very general exactly in which step you had not result?