DEPC treatment - FAQS.TIPS

More Shelley Kummer's questions See All

Nasal microsome protein concentration?

Hello, If anyone has extracted microsomes from either rat and/or human nasal tissue, could you tell me what the total microsomal protein concentration is (mg/g)? This never seems to be included in...

07 August 2016 7,994 1 View

Vectors

Hi all, I was wondering if anyone has ever come across a commercially available promoterless lacz vector for bacteria (ie I want to clone in a putative bacterial promoter and assay for lacz...

09 October 2010 5,186 1 View

Cell encapsulation in Alginate

Hi Everyone, we have been trying to embed cells in alginate that we will mechanically stimulate and then want to extract RNA and protein downstream to look at gene expression. We have been having...

08 September 2010 1,655 7 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

Do you consider undergoing a structure literature review / Meta analysis and getting numerical findings sort of quantitative method?

I am looking for a classification of the research methods included in literature review papers or Meta analysis ones .

22 February 2021 1,131 4 View

Can I do two kinds of mixed method in explanatory research?

Dear distinguish professors and scholars, I have a research in measuring two kinds: think tank capacity and research impact on policy. Can I do two kinds of mixed methods in order to find, "Is...

20 February 2021 6,897 1 View

How to analyse a (perceptual) study using differing/comparable sets of stimuli?

I plan on running an online perceptual experiment that has, let's say, 100 unique video stimuli altogether. Since these are too many to be rated by every participants, I would like to present the...

15 February 2021 7,444 2 View

How to analyse a split-plot design in stata ?

I have run an split-plot design experiment evaluating the efficacy of a treatment (with 3 whole plots and 4 sub-plots), and I am planning to analyse the collected data in Stata. However, I do...

14 February 2021 7,800 7 View

Can I use a likert survey for criterion sampling in a qualitative study or would that make it mixed methods?

I am doing a phenomenographic study on the reading experiences of avid v. Neutral v. Non-Readers. If I use a likert survey to criterion sample for participants, does that make it a mixed method...

09 February 2021 8,594 3 View

How to create the context-mechanism-outcome configuration (CMOc) in the realist methodology?

In the realist methodology (especially by Raymond Pawson et al), the CMOc is a common structure to explain what works, for whom, in what circumstances, and why? I'm always asked about the...

08 February 2021 2,792 1 View

Recommendations for sample size in visual qualitative research (using children's drawings)?

Hi everyone! Asking for a little help, if I may. I am working on a research project, where I aim to analyse children's drawings about Covid-19. I am fairly new to using visual data in qualitative...

07 February 2021 9,530 3 View

How do I create a categorical variable, level of exposure, and a dummy dichotomous, yes-no, variable for the Harvard Trauma Questionnaire Part 1?

I am using the Harvard Trauma Questionnaire (HTQ) Trauma Events scale (original version) that has 17 items (17 different traumatic events) with 4 response options, indicating 4 levels of exposure:...

04 February 2021 1,509 3 View

How to report path analysis / SEM with Yuan-Bentler correction from R output?

Dear all, I am currently writing my master thesis where I am analysizing my data using path analysis and SEM in R (lavaan package). Now that I like to write up my results, I am struggling with...

04 February 2021 8,993 2 View

Ram Nivas Ahirwar

Yes, It is ok to use that DEPC water. It never hinders in reactions.

Basak Ozturk

Hhhmm... I would not agree there. If you are going to use the water for molecular biology, you should buy nuclease free water (from Ambion or so) which is Gamma-radiated instead of DEPC treated. Trace amounts of DEPC will otherwise inhibit reactions -especially cDNA synthesis. here are some options, and you can read more technical data on the Ambion web site as well:

http://www.ambion.com/catalog/CatNum.php?AM9932

Shelley Kummer

Yes, that is what I thought too. ---