How can I solve my False-Positive issues with the SARS-CoV-2 RPA amplification ?

Here, I want to ask about my issue with the SARS-CoV-2 RPA amplification False-Positive results. I tried two different sets of primers. The first sets of primers are 29 nt producing 200 bp...

20 December 2020 2,091 3 View

How can I check the correlation between a group of features and one feature?

Hello all, I have a dataset that contains some pressure meters in a water distribution network and all valves of the network. Since I do not have the graph of the water distribution network, I...

30 October 2020 2,333 4 View

Hi everyone, Can anyone kindly help me for analyzing the attached FTIR spectra?

It's an organic material used as a dispersant for refractory concretes. I had some search and found that it can be an acrylamide based compound but I am not sure yet. The datasheet supplied by the...

04 October 2020 994 9 View

Why am I getting multiple bands in western blot (in Isolating adhesion complexes) ?

Hello everyone, I am isolating adhesion complexes and wanted to confirm the presence of a specific protein in the isolated complex. When I performed western bott, I got a band at the right size...

19 September 2020 538 7 View

How can we optimize a water distribution system if we do not have the zones' topology (pipes graph )?

Hello everyone, My issue is about a water distribution system that I am working on a zone of the system where does not exist any plan for the place of pipes. However, we have the place of...

14 September 2020 5,978 6 View

Could we 3-D print brain, spine, heart, lungs, whole body, thought process?

https://www.cnnphilippines.com/world/2020/1/24/Voice-of-3,000-year-old-mummy-reproduced-.html?fbclid=IwAR3sQKGlP6Aj5XiPpXY0eJFsgQafIu7_sMyP4GBfiQViP4xcejM3krwmog4

25 January 2020 9,427 1 View

Oceanic controlled continental fragmentation?

Oceanic Waters center of gravitation should be attracted to the center of gravity of the Earth. However, because the water of the oceans is different from the solid mass of the Earth, the water of...

16 January 2020 177 9 View

What's the best protocol for diluting DMSO when thawing the primary human epithelial cells?

Hi, I have some problem with the viability of my primary epithelial cells after thawing. I have used two protocols (centrifuging or only diluting 10 times more and changing the media the day...

03 October 2019 7,990 6 View

No title

25 March 2019 4,032 3 View

What is the relation between AIDS and non-AIDS diseases among HIV positive patients?

Among HIV positive patients, is it possible that non-AIDS diseases alter the risk of developing AIDS and vice versa? For a HIV positive patient who has experienced a non-AIDS disease, Does the...

24 January 2019 1,044 4 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

How do I determine the degree of polymerization of PEG 400 based on NMR integration?

Hello, I am working on the photocatalytic degradation of PEG 400. I would like to know if it's possible to determine the degree on polymerization based on NMR integration, or by the MW of the...

02 March 2021 6,186 3 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Is there any published or unpublished statistics on dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, US, UK or any other country?

Are there any published or unpublished statistics or papers on the dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, USA, UK, or any other country where vaccination has started at...

28 February 2021 4,237 1 View

How to include original NMR data in a manuscript?

hello. i am junior researcher. I received a comment from a reviewer that he could not find NMR data in my submitted paper. I had included the NMR graph with explanation and formula to calculate...

27 February 2021 734 4 View

Steps to calculate activation energy by coats & redfern method?

I'm a chemistry student and I'm researching the kinetic degradation of mixed plastic waste. I'm having trouble calculating the activation energy by the coats & Redfern method. The data that...

24 February 2021 974 1 View

Any Differences in COVID-19 vaccines?

Are there any differences (outside of the clinical trials that led to their EUA) observed in the rate of infection among those who have received the Pfizer vs Moderna vaccine since the mass...

22 February 2021 9,727 6 View

How to find natural frequency of a bridge from vehicle induced vibrations?

i have frequencies of bridge that are effected by mass of vehicle. the problem is when any vehicle pass the bridge it would add the its mass to the bridge mass ultimately mass of the bridge will...

22 February 2021 10,004 3 View

What does it require to be ready for e-Commerce in developing countries?

Most developing countries are not ready for e-Commerce? What dimensions and indicators are there to measure e-commerce readiness of a nation? Please share framework or model and evaluation...

22 February 2021 8,132 6 View