Hello everyone

I understand that the most accepted model to investigate DC development (differentiation and maturation) in vitro is the isolation of BMP's from mice and stimulate them with FLT3L/GM-CSF etc or for maturation with LPS. My question though is,  how well does this translate to an invitro system, where the exposure is a not as specific but is affected by a vast repertoire of  antigens ? Also are there any other models which have been suggested as more acceptable than the BMDC protocol.

This topic is striclty restricted to the steady state development of DC's and not the inflammatory response based development.

Thanks in advance

Anirudh