Having a hard time getting a decent slide out of rat spinal cord sections ive mounted in paraffin. There's tiny streaks/tears in the section it self but not the surrounding wax. Here's the protocol i've been following:

50% alcohol: 30 mins

70% alcohol: 30 mins

90% alcohol: 30 mins

100% alcohol: 30 mins x 2

xylene: 30 mins x 2

wax overnight at 65*C

Does anyone have any suggestions that will fix the small tears in the sample?

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